Coding

Part:BBa_K2074101:Design

Designed by: Hao Feng   Group: iGEM16_FAFU-CHINA   (2016-10-11)


Cry4Aa from Bacillus thuringiensis serovar israelensis strain BRC-LLP29


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 1766
    Illegal SpeI site found at 612
    Illegal SpeI site found at 2502
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 1766
    Illegal SpeI site found at 612
    Illegal SpeI site found at 2502
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 1766
    Illegal BglII site found at 912
    Illegal XhoI site found at 980
    Illegal XhoI site found at 1799
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 1766
    Illegal SpeI site found at 612
    Illegal SpeI site found at 2502
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 1766
    Illegal SpeI site found at 612
    Illegal SpeI site found at 2502
    Illegal NgoMIV site found at 2625
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

3


Source

Bacillus thuringiensis serovar israelensis strain BRC-LLP29 putative cry4A gene, complete cds；GenBank: JQ228565.1

References